LOCUS       Exported                4379 bp ds-DNA    circular SYN 17-OCT-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    piG0067_logic_bxb1_BUFFER-sfGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Daniel Gerngross
  TITLE     Direct Submission
  JOURNAL   Exported 17 Oct 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      1..126
                     /note="T0"
     terminator      27..121
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     promoter        165..199
                     /note="J23100"
     misc_feature    200..249
                     /note="attB Bxb1"
     misc_feature    224..225
                     /note="conserved"
     terminator      250..378
                     /note="B0015"
                     /note="directional double term"
     terminator      258..329
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      345..372
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    complement(379..436)
                     /note="attP Bxb1"
     misc_feature    407..408
                     /note="conserved"
     misc_feature    437..456
                     /note="35"
                     /note="35 nt upstream of ATG from bla gene from pUC18 (bla 
                     RBS) --> introduction of XbaI site for library cloning"
     CDS             472..1197
                     /codon_start=1
                     /note="sfGFP"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYKRP*"
     CDS             1192..1194
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /note="mRFP1"
                     /translation=""
     terminator      1209..1295
                     /gene="Escherichia coli rrnB"
                     /note="T1"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      1410..2181
                     /note="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     rep_origin      2059..2844
                     /note="pBBR1"
     CDS             2182..2844
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /note="pBBR1 Rep"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     oriT            2855..2963
                     /note="incP origin of transfer"
     CDS             complement(3109..4299)
                     /note="TetR"
ORIGIN
        1 aaatcgtaat tattggggac ccctggattc tcaccaataa aaaacgcccg gcggcaaccg
       61 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
      121 ccaagactag tcgccagggt tttcccagtc acgacgcggc cgcattgacg gctagctcag
      181 tcctaggtac agtgctagct cggccggctt gtcgacgacg gcggtctccg tcgtcaggat
      241 catccgggcc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      301 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      361 ggcctttctg cgtttatagt cggggtttgt accgtacacc actgagaccg cggtggttga
      421 ccagacaaac cacgacgata aatgcttcaa taatatgagg aggtgatcta gatgagcaaa
      481 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
      541 gggcacaaat tttctgtccg tggagagggt gaaggtgatg ctacaaacgg aaaactcacc
      601 cttaaattta tttgcactac tggaaaacta cctgttccgt ggccaacact tgtcactact
      661 ctgacctatg gtgttcaatg cttttcccgt tatccggatc acatgaaacg gcatgacttt
      721 ttcaagagtg ccatgcccga aggttatgta caggaacgca ctatatcttt caaagatgac
      781 gggacctaca agacgcgtgc tgaagtcaag tttgaaggtg atacccttgt taatcgtatc
      841 gagttaaagg gtattgattt taaagaagat ggaaacattc ttggacacaa actcgagtac
      901 aactttaact cacacaatgt atacatcacg gcagacaaac aaaagaatgg aatcaaagct
      961 aacttcaaaa ttcgccacaa cgttgaagat ggttccgttc aactagcaga ccattatcaa
     1021 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtcgaca
     1081 caatctgtcc tttcgaaaga tcccaacgaa aagcgtgacc acatggtcct tcttgagttt
     1141 gtaactgctg ctgggattac acatggcatg gatgagctct acaaaaggcc ttaataaatt
     1201 aaaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1261 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gccctagaca gctgggcgcg
     1321 ccccccctac gggcttgctc tccgggcttc gccctgcgcg gtcgctgcgc tcccttgcca
     1381 gcccgtggat atgtggacga tggccgcgag cggccaccgg ctggctcgct tcgctcggcc
     1441 cgtggacaac cctgctggac aagctgatgg acaggctgcg cctgcccacg agcttgacca
     1501 cagggattgc ccaccggcta cccagccttc gaccacatac ccaccggctc caactgcgcg
     1561 gcctgcggcc ttgccccatc aattttttta attttctctg gggaaaagcc tccggcctgc
     1621 ggcctgcgcg cttcgcttgc cggttggaca ccaagtggaa ggcgggtcaa ggctcgcgca
     1681 gcgaccgcgc agcggcttgg ccttgacgcg cctggaacga cccaagccta tgcgagtggg
     1741 ggcagtcgaa gggcgaagcc cgcccgcctg ccccccgagc ctcacggcgg cgagtgcggg
     1801 ggttccaagg gggcagcgcc accttgggca aggccgaagg ccgcgcagtc gatcaacaag
     1861 ccccggaggg gccacttttt gccggagggg gagccgcgcc gaaggcgtgg gggaaccccg
     1921 caggggtgcc cttctttggg caccaaagaa ctagatatag ggcgaaatgc gaaagactta
     1981 aaaatcaaca acttaaaaaa ggggggtacg caacagctca ttgcggcacc ccccgcaata
     2041 gctcattgcg taggttaaag aaaatctgta attgactgcc acttttacgc aacgcataat
     2101 tgttgtcgcg ctgccgaaaa gttgcagctg attgcgcatg gtgccgcaac cgtgcggcac
     2161 ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat tcaagccaag
     2221 aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg ggccgggctt
     2281 attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca gatgggccac
     2341 cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg ttctttgcgg
     2401 acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt cgtgaagctc
     2461 aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg gggccagccc
     2521 cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca cgacgaccag
     2581 gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta tccgggcgag
     2641 cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat tccgggcatg
     2701 gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg gcagcagcgc
     2761 ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga gccgccgaca
     2821 cgggtaacgc tgccgcgccg gtagggccgg cctacggcca gcctcgcaga gcaggattcc
     2881 cgttgagcac cgccaggtgc gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg
     2941 ggcctacttc acctatcctg cccggctgac gccgttggat acaccaagga aagtctacac
     3001 gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg
     3061 ctataatgac cccgaagcag ggttatgcag cggaaaagga caattgtctc aggtcgaggt
     3121 ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc
     3181 tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat
     3241 cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc
     3301 ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc
     3361 gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag
     3421 caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa
     3481 acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac
     3541 cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac
     3601 ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc
     3661 ggccacaatg gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa
     3721 gggcatcgga cggcgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag
     3781 gttgaggccg ttgagcaccg ccgccgcaag gaatggtgcg tgcagggaga tggcgcccaa
     3841 cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc
     3901 gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc
     3961 acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagagaatcc acaggacggg
     4021 tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg
     4081 gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa
     4141 cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc
     4201 ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac
     4261 ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta
     4321 gcaatttaac tgtgataaac taccgcatta cagtttatcg atgataagct gtcaaattt
//
